Regulated expression of the Alcaligenes eutrophus pha biosynthesis genes in Escherichia coli
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چکیده
منابع مشابه
Regulated expression of the Alcaligenes eutrophus pha biosynthesis genes in Escherichia coli.
A novel poly-beta-hydroxybutyrate (PHB) production system in which the expression and gene dosage of the Alcaligenes eutrophus pha biosynthetic operon were effectively regulated by cultivation temperature was constructed in Escherichia coli. The pha operon was fused to the negatively regulated tac promoter and cloned into a vector in which the copy number is temperature dependent. A two-phase p...
متن کاملExpression of the Escherichia coli pfkA gene in Alcaligenes eutrophus and in other gram-negative bacteria.
The Escherichia coli pfkA gene has been cloned in the non-self-transmissible vector pVK101 from hybrid plasmids obtained from the Clarke and Carbon clone bank, resulting in the plasmids pAS300 and pAS100; the latter plasmid also encoded the E. coli tpi gene. These plasmids were transferred by conjugation to mutants of Alcaligenes eutrophus which are unable to grow on fructose and gluconate due ...
متن کاملThe Alcaligenes eutrophus protein HoxN mediates nickel transport in Escherichia coli.
HoxN, an integral membrane protein with seven transmembrane helices and a molecular mass of 33.1 kDa, is involved in high-affinity nickel transport in Alcaligenes eutrophus H16. From genetic analyses, it has been concluded that HoxN is a single-component ion carrier. To investigate this assumption, hoxN was introduced into Escherichia coli. The recombinant strain showed significantly enhanced n...
متن کامل-regulated genes in Escherichia coli
Accepted 9 March, 2006. *For correspondence. E-mail pjkiley @wisc.edu; Tel. ( + 1) 608 262 6632; Fax ( + 1) 608 262 5253. Present address: † The Burnham Institute, La Jolla, CA 92037, USA; ‡ Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706, USA. IscR-dependent gene expression links iron-sulphur cluster assembly to the control of O 2 -regulated genes in Escherichi...
متن کاملChromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus.
Genes coding for phosphoribulokinase (PRK), a key enzyme of the Calvin cycle, were localized in the genome of the chemoautotroph Alcaligenes eutrophus. The NH2-terminal sequence of the PRK subunit was determined. With a synthetic oligodeoxynucleotide probe complementary to a portion of this sequence, hybridization analysis revealed PRK genes to be located on both the chromosome and the megaplas...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1995
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.61.4.1391-1398.1995